Composite

Part:BBa_K551000

Designed by: Laurence Van Melderen   Group: iGEM11_ULB-Brussels   (2011-09-19)

FRT-CM-FRT

This part is composed of two mutated Flipase Recognition Targets (FRT') flanking the Chloramphenicol resistance gene. The mutation removes the XbaI restriction site from the canonical FRT sequence. It is similar to BBa_J61025 but without the scar between the first FRT' and CmR.

The two FRT' sequences allow the excision of the flanked sequence by the Flipase recombinase.

It's been tested with success for resistance to Chloramphenicol and excision per the pINDEL plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination/flp
Parameters
resistance20 �g/ml